Review



ubc12  (Boston Biochem)


Bioz Verified Symbol Boston Biochem is a verified supplier  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
    • 94
      Buy from Supplier

    Structured Review

    Boston Biochem ubc12
    Ubc12, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 94/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+656/bio_rxiv__2023__02__06__527237-268-51-56?v=Boston+Biochem
    Average 94 stars, based on 5 article reviews
    ubc12 - by Bioz Stars, 2026-07
    94/100 stars

    Images



    Similar Products

    ube2m  (R&D Systems)
    94
    R&D Systems ube2m
    Ube2m, supplied by R&D Systems, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+656/bio_rxiv__64898__2026__03__02__709011-268-31-32?v=R%26D+Systems
    Average 94 stars, based on 1 article reviews
    ube2m - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    e2 ube2m e2 656 100 r d  (R&D Systems Hematology)
    94
    R&D Systems Hematology e2 ube2m e2 656 100 r d
    (A) Co-immunoprecipitation of BCR-ABL and RAPSYN in leukemic cells. (B) Immunoblot of GST and His after immunoprecipitation of His or GST in HEK293T cells transfected with His-tagged BCR-ABL and GST-tagged RAPSYN. (C) Immunoblot of GST and His following GST pull-down after in vitro incubation of purified His-tagged BCR-ABL and GST or GST-tagged RAPSYN. (D) His-immunoblot of GST immunoprecipitates from HEK293T cells transfected with GST-tagged RAPSYN alone or in combination with His-tagged full-length or truncated BCR-ABL (Δ1: aa 1-927, Δ2: aa 928-2047). (E) Analysis of BCR-ABL neddylation levels in leukemic cells. (F) Analysis of BCR-ABL neddylation levels in leukemic cells treated with MLN4924 or DMSO for 24 h. (G) HA-immunoblot of His-immunoprecipitate from HEK293T cells transfected with His-tagged BCR-ABL and HA-tagged NEDD8 or NEDD8 ΔGG. (H) HA-immunoblot of the His-immunoprecipitate from HEK293T cells transfected with the indicated constructs. (I) Analysis of BCR-ABL neddylation levels in K562 WT, RAPSYN KO and RAPSYN KO with exogenous expression of a RAPSN cDNA cells. (J) HA-immunoblot after immunoprecipitation of His in HEK293T cells transfected with His-tagged BCR-ABL, HA-tagged NEDD8, GFP-tagged WT RAPSYN or C366A RAPSYN. (K) Assessment of BCR-ABL neddylation by RAPSYN in vitro. Purified RAPSYN and BCR-ABL were incubated with APPBP1/UBA3, <t>UBE2M</t> or NEDD8 in in vitro neddylation assay. (L) Analysis of BCR-ABL neddylation levels in excised tumor xenografts from . (M) Verification of BCR-ABL neddylation sites in HEK293T cells transfected with indicated constructs.
    E2 Ube2m E2 656 100 R D, supplied by R&D Systems Hematology, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+656/bio_rxiv__2023__05__24__542110-170-18-21?v=R%26D+Systems+Hematology
    Average 94 stars, based on 1 article reviews
    e2 ube2m e2 656 100 r d - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    ubc12  (Boston Biochem)
    94
    Boston Biochem ubc12
    (A) Co-immunoprecipitation of BCR-ABL and RAPSYN in leukemic cells. (B) Immunoblot of GST and His after immunoprecipitation of His or GST in HEK293T cells transfected with His-tagged BCR-ABL and GST-tagged RAPSYN. (C) Immunoblot of GST and His following GST pull-down after in vitro incubation of purified His-tagged BCR-ABL and GST or GST-tagged RAPSYN. (D) His-immunoblot of GST immunoprecipitates from HEK293T cells transfected with GST-tagged RAPSYN alone or in combination with His-tagged full-length or truncated BCR-ABL (Δ1: aa 1-927, Δ2: aa 928-2047). (E) Analysis of BCR-ABL neddylation levels in leukemic cells. (F) Analysis of BCR-ABL neddylation levels in leukemic cells treated with MLN4924 or DMSO for 24 h. (G) HA-immunoblot of His-immunoprecipitate from HEK293T cells transfected with His-tagged BCR-ABL and HA-tagged NEDD8 or NEDD8 ΔGG. (H) HA-immunoblot of the His-immunoprecipitate from HEK293T cells transfected with the indicated constructs. (I) Analysis of BCR-ABL neddylation levels in K562 WT, RAPSYN KO and RAPSYN KO with exogenous expression of a RAPSN cDNA cells. (J) HA-immunoblot after immunoprecipitation of His in HEK293T cells transfected with His-tagged BCR-ABL, HA-tagged NEDD8, GFP-tagged WT RAPSYN or C366A RAPSYN. (K) Assessment of BCR-ABL neddylation by RAPSYN in vitro. Purified RAPSYN and BCR-ABL were incubated with APPBP1/UBA3, <t>UBE2M</t> or NEDD8 in in vitro neddylation assay. (L) Analysis of BCR-ABL neddylation levels in excised tumor xenografts from . (M) Verification of BCR-ABL neddylation sites in HEK293T cells transfected with indicated constructs.
    Ubc12, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+656/bio_rxiv__2023__02__06__527237-268-51-56?v=Boston+Biochem
    Average 94 stars, based on 1 article reviews
    ubc12 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    ubiquitination e1  (Boston Biochem)
    94
    Boston Biochem ubiquitination e1
    (A) Co-immunoprecipitation of BCR-ABL and RAPSYN in leukemic cells. (B) Immunoblot of GST and His after immunoprecipitation of His or GST in HEK293T cells transfected with His-tagged BCR-ABL and GST-tagged RAPSYN. (C) Immunoblot of GST and His following GST pull-down after in vitro incubation of purified His-tagged BCR-ABL and GST or GST-tagged RAPSYN. (D) His-immunoblot of GST immunoprecipitates from HEK293T cells transfected with GST-tagged RAPSYN alone or in combination with His-tagged full-length or truncated BCR-ABL (Δ1: aa 1-927, Δ2: aa 928-2047). (E) Analysis of BCR-ABL neddylation levels in leukemic cells. (F) Analysis of BCR-ABL neddylation levels in leukemic cells treated with MLN4924 or DMSO for 24 h. (G) HA-immunoblot of His-immunoprecipitate from HEK293T cells transfected with His-tagged BCR-ABL and HA-tagged NEDD8 or NEDD8 ΔGG. (H) HA-immunoblot of the His-immunoprecipitate from HEK293T cells transfected with the indicated constructs. (I) Analysis of BCR-ABL neddylation levels in K562 WT, RAPSYN KO and RAPSYN KO with exogenous expression of a RAPSN cDNA cells. (J) HA-immunoblot after immunoprecipitation of His in HEK293T cells transfected with His-tagged BCR-ABL, HA-tagged NEDD8, GFP-tagged WT RAPSYN or C366A RAPSYN. (K) Assessment of BCR-ABL neddylation by RAPSYN in vitro. Purified RAPSYN and BCR-ABL were incubated with APPBP1/UBA3, <t>UBE2M</t> or NEDD8 in in vitro neddylation assay. (L) Analysis of BCR-ABL neddylation levels in excised tumor xenografts from . (M) Verification of BCR-ABL neddylation sites in HEK293T cells transfected with indicated constructs.
    Ubiquitination E1, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+656/pm34118280-125-30-34?v=Boston+Biochem
    Average 94 stars, based on 1 article reviews
    ubiquitination e1 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    ubiquitination e2  (Boston Biochem)
    94
    Boston Biochem ubiquitination e2
    (A) Co-immunoprecipitation of BCR-ABL and RAPSYN in leukemic cells. (B) Immunoblot of GST and His after immunoprecipitation of His or GST in HEK293T cells transfected with His-tagged BCR-ABL and GST-tagged RAPSYN. (C) Immunoblot of GST and His following GST pull-down after in vitro incubation of purified His-tagged BCR-ABL and GST or GST-tagged RAPSYN. (D) His-immunoblot of GST immunoprecipitates from HEK293T cells transfected with GST-tagged RAPSYN alone or in combination with His-tagged full-length or truncated BCR-ABL (Δ1: aa 1-927, Δ2: aa 928-2047). (E) Analysis of BCR-ABL neddylation levels in leukemic cells. (F) Analysis of BCR-ABL neddylation levels in leukemic cells treated with MLN4924 or DMSO for 24 h. (G) HA-immunoblot of His-immunoprecipitate from HEK293T cells transfected with His-tagged BCR-ABL and HA-tagged NEDD8 or NEDD8 ΔGG. (H) HA-immunoblot of the His-immunoprecipitate from HEK293T cells transfected with the indicated constructs. (I) Analysis of BCR-ABL neddylation levels in K562 WT, RAPSYN KO and RAPSYN KO with exogenous expression of a RAPSN cDNA cells. (J) HA-immunoblot after immunoprecipitation of His in HEK293T cells transfected with His-tagged BCR-ABL, HA-tagged NEDD8, GFP-tagged WT RAPSYN or C366A RAPSYN. (K) Assessment of BCR-ABL neddylation by RAPSYN in vitro. Purified RAPSYN and BCR-ABL were incubated with APPBP1/UBA3, <t>UBE2M</t> or NEDD8 in in vitro neddylation assay. (L) Analysis of BCR-ABL neddylation levels in excised tumor xenografts from . (M) Verification of BCR-ABL neddylation sites in HEK293T cells transfected with indicated constructs.
    Ubiquitination E2, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+656/pm34118280-125-40-43?v=Boston+Biochem
    Average 94 stars, based on 1 article reviews
    ubiquitination e2 - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    paper n a e1 uba1 rapé lab n a ube2r1 rapé lab n a uba3 boston biochem  (Boston Biochem)
    94
    Boston Biochem paper n a e1 uba1 rapé lab n a ube2r1 rapé lab n a uba3 boston biochem
    (A) Co-immunoprecipitation of BCR-ABL and RAPSYN in leukemic cells. (B) Immunoblot of GST and His after immunoprecipitation of His or GST in HEK293T cells transfected with His-tagged BCR-ABL and GST-tagged RAPSYN. (C) Immunoblot of GST and His following GST pull-down after in vitro incubation of purified His-tagged BCR-ABL and GST or GST-tagged RAPSYN. (D) His-immunoblot of GST immunoprecipitates from HEK293T cells transfected with GST-tagged RAPSYN alone or in combination with His-tagged full-length or truncated BCR-ABL (Δ1: aa 1-927, Δ2: aa 928-2047). (E) Analysis of BCR-ABL neddylation levels in leukemic cells. (F) Analysis of BCR-ABL neddylation levels in leukemic cells treated with MLN4924 or DMSO for 24 h. (G) HA-immunoblot of His-immunoprecipitate from HEK293T cells transfected with His-tagged BCR-ABL and HA-tagged NEDD8 or NEDD8 ΔGG. (H) HA-immunoblot of the His-immunoprecipitate from HEK293T cells transfected with the indicated constructs. (I) Analysis of BCR-ABL neddylation levels in K562 WT, RAPSYN KO and RAPSYN KO with exogenous expression of a RAPSN cDNA cells. (J) HA-immunoblot after immunoprecipitation of His in HEK293T cells transfected with His-tagged BCR-ABL, HA-tagged NEDD8, GFP-tagged WT RAPSYN or C366A RAPSYN. (K) Assessment of BCR-ABL neddylation by RAPSYN in vitro. Purified RAPSYN and BCR-ABL were incubated with APPBP1/UBA3, <t>UBE2M</t> or NEDD8 in in vitro neddylation assay. (L) Analysis of BCR-ABL neddylation levels in excised tumor xenografts from . (M) Verification of BCR-ABL neddylation sites in HEK293T cells transfected with indicated constructs.
    Paper N A E1 Uba1 Rapé Lab N A Ube2r1 Rapé Lab N A Uba3 Boston Biochem, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+656/pm34562363-244-46-57?v=Boston+Biochem
    Average 94 stars, based on 1 article reviews
    paper n a e1 uba1 rapé lab n a ube2r1 rapé lab n a uba3 boston biochem - by Bioz Stars, 2026-07
    94/100 stars
    		  Buy from Supplier
    e2 656  (Boston Biochem)
    93
    Boston Biochem e2 656
    (A) Co-immunoprecipitation of BCR-ABL and RAPSYN in leukemic cells. (B) Immunoblot of GST and His after immunoprecipitation of His or GST in HEK293T cells transfected with His-tagged BCR-ABL and GST-tagged RAPSYN. (C) Immunoblot of GST and His following GST pull-down after in vitro incubation of purified His-tagged BCR-ABL and GST or GST-tagged RAPSYN. (D) His-immunoblot of GST immunoprecipitates from HEK293T cells transfected with GST-tagged RAPSYN alone or in combination with His-tagged full-length or truncated BCR-ABL (Δ1: aa 1-927, Δ2: aa 928-2047). (E) Analysis of BCR-ABL neddylation levels in leukemic cells. (F) Analysis of BCR-ABL neddylation levels in leukemic cells treated with MLN4924 or DMSO for 24 h. (G) HA-immunoblot of His-immunoprecipitate from HEK293T cells transfected with His-tagged BCR-ABL and HA-tagged NEDD8 or NEDD8 ΔGG. (H) HA-immunoblot of the His-immunoprecipitate from HEK293T cells transfected with the indicated constructs. (I) Analysis of BCR-ABL neddylation levels in K562 WT, RAPSYN KO and RAPSYN KO with exogenous expression of a RAPSN cDNA cells. (J) HA-immunoblot after immunoprecipitation of His in HEK293T cells transfected with His-tagged BCR-ABL, HA-tagged NEDD8, GFP-tagged WT RAPSYN or C366A RAPSYN. (K) Assessment of BCR-ABL neddylation by RAPSYN in vitro. Purified RAPSYN and BCR-ABL were incubated with APPBP1/UBA3, <t>UBE2M</t> or NEDD8 in in vitro neddylation assay. (L) Analysis of BCR-ABL neddylation levels in excised tumor xenografts from . (M) Verification of BCR-ABL neddylation sites in HEK293T cells transfected with indicated constructs.
    E2 656, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+656/pm33609460-236-309-306?v=Boston+Biochem
    Average 93 stars, based on 1 article reviews
    e2 656 - by Bioz Stars, 2026-07
    93/100 stars
    		  Buy from Supplier
    ube2m (e2, e2-656)  (Boston Biochem)
    90
    Boston Biochem ube2m (e2, e2-656)
    (A) Co-immunoprecipitation of BCR-ABL and RAPSYN in leukemic cells. (B) Immunoblot of GST and His after immunoprecipitation of His or GST in HEK293T cells transfected with His-tagged BCR-ABL and GST-tagged RAPSYN. (C) Immunoblot of GST and His following GST pull-down after in vitro incubation of purified His-tagged BCR-ABL and GST or GST-tagged RAPSYN. (D) His-immunoblot of GST immunoprecipitates from HEK293T cells transfected with GST-tagged RAPSYN alone or in combination with His-tagged full-length or truncated BCR-ABL (Δ1: aa 1-927, Δ2: aa 928-2047). (E) Analysis of BCR-ABL neddylation levels in leukemic cells. (F) Analysis of BCR-ABL neddylation levels in leukemic cells treated with MLN4924 or DMSO for 24 h. (G) HA-immunoblot of His-immunoprecipitate from HEK293T cells transfected with His-tagged BCR-ABL and HA-tagged NEDD8 or NEDD8 ΔGG. (H) HA-immunoblot of the His-immunoprecipitate from HEK293T cells transfected with the indicated constructs. (I) Analysis of BCR-ABL neddylation levels in K562 WT, RAPSYN KO and RAPSYN KO with exogenous expression of a RAPSN cDNA cells. (J) HA-immunoblot after immunoprecipitation of His in HEK293T cells transfected with His-tagged BCR-ABL, HA-tagged NEDD8, GFP-tagged WT RAPSYN or C366A RAPSYN. (K) Assessment of BCR-ABL neddylation by RAPSYN in vitro. Purified RAPSYN and BCR-ABL were incubated with APPBP1/UBA3, <t>UBE2M</t> or NEDD8 in in vitro neddylation assay. (L) Analysis of BCR-ABL neddylation levels in excised tumor xenografts from . (M) Verification of BCR-ABL neddylation sites in HEK293T cells transfected with indicated constructs.
    Ube2m (E2, E2 656), supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+656/pm32941802-550-7-17?v=Boston+Biochem
    Average 90 stars, based on 1 article reviews
    ube2m (e2, e2-656) - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier
    ubc12 e2-656  (Boston Biochem)
    90
    Boston Biochem ubc12 e2-656
    (A) Co-immunoprecipitation of BCR-ABL and RAPSYN in leukemic cells. (B) Immunoblot of GST and His after immunoprecipitation of His or GST in HEK293T cells transfected with His-tagged BCR-ABL and GST-tagged RAPSYN. (C) Immunoblot of GST and His following GST pull-down after in vitro incubation of purified His-tagged BCR-ABL and GST or GST-tagged RAPSYN. (D) His-immunoblot of GST immunoprecipitates from HEK293T cells transfected with GST-tagged RAPSYN alone or in combination with His-tagged full-length or truncated BCR-ABL (Δ1: aa 1-927, Δ2: aa 928-2047). (E) Analysis of BCR-ABL neddylation levels in leukemic cells. (F) Analysis of BCR-ABL neddylation levels in leukemic cells treated with MLN4924 or DMSO for 24 h. (G) HA-immunoblot of His-immunoprecipitate from HEK293T cells transfected with His-tagged BCR-ABL and HA-tagged NEDD8 or NEDD8 ΔGG. (H) HA-immunoblot of the His-immunoprecipitate from HEK293T cells transfected with the indicated constructs. (I) Analysis of BCR-ABL neddylation levels in K562 WT, RAPSYN KO and RAPSYN KO with exogenous expression of a RAPSN cDNA cells. (J) HA-immunoblot after immunoprecipitation of His in HEK293T cells transfected with His-tagged BCR-ABL, HA-tagged NEDD8, GFP-tagged WT RAPSYN or C366A RAPSYN. (K) Assessment of BCR-ABL neddylation by RAPSYN in vitro. Purified RAPSYN and BCR-ABL were incubated with APPBP1/UBA3, <t>UBE2M</t> or NEDD8 in in vitro neddylation assay. (L) Analysis of BCR-ABL neddylation levels in excised tumor xenografts from . (M) Verification of BCR-ABL neddylation sites in HEK293T cells transfected with indicated constructs.
    Ubc12 E2 656, supplied by Boston Biochem, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/e2+656/pmc08179036__SC___012___D0SC00958J___s001-151-21-36?v=Boston+Biochem
    Average 90 stars, based on 1 article reviews
    ubc12 e2-656 - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    (A) Co-immunoprecipitation of BCR-ABL and RAPSYN in leukemic cells. (B) Immunoblot of GST and His after immunoprecipitation of His or GST in HEK293T cells transfected with His-tagged BCR-ABL and GST-tagged RAPSYN. (C) Immunoblot of GST and His following GST pull-down after in vitro incubation of purified His-tagged BCR-ABL and GST or GST-tagged RAPSYN. (D) His-immunoblot of GST immunoprecipitates from HEK293T cells transfected with GST-tagged RAPSYN alone or in combination with His-tagged full-length or truncated BCR-ABL (Δ1: aa 1-927, Δ2: aa 928-2047). (E) Analysis of BCR-ABL neddylation levels in leukemic cells. (F) Analysis of BCR-ABL neddylation levels in leukemic cells treated with MLN4924 or DMSO for 24 h. (G) HA-immunoblot of His-immunoprecipitate from HEK293T cells transfected with His-tagged BCR-ABL and HA-tagged NEDD8 or NEDD8 ΔGG. (H) HA-immunoblot of the His-immunoprecipitate from HEK293T cells transfected with the indicated constructs. (I) Analysis of BCR-ABL neddylation levels in K562 WT, RAPSYN KO and RAPSYN KO with exogenous expression of a RAPSN cDNA cells. (J) HA-immunoblot after immunoprecipitation of His in HEK293T cells transfected with His-tagged BCR-ABL, HA-tagged NEDD8, GFP-tagged WT RAPSYN or C366A RAPSYN. (K) Assessment of BCR-ABL neddylation by RAPSYN in vitro. Purified RAPSYN and BCR-ABL were incubated with APPBP1/UBA3, UBE2M or NEDD8 in in vitro neddylation assay. (L) Analysis of BCR-ABL neddylation levels in excised tumor xenografts from . (M) Verification of BCR-ABL neddylation sites in HEK293T cells transfected with indicated constructs.

    Journal: bioRxiv

    Article Title: RAPSYN-Mediated Neddylation of BCR-ABL Alternatively Determines the Fate of Philadelphia Chromosome-positive Leukemia

    doi: 10.1101/2023.05.24.542110

    Figure Lengend Snippet: (A) Co-immunoprecipitation of BCR-ABL and RAPSYN in leukemic cells. (B) Immunoblot of GST and His after immunoprecipitation of His or GST in HEK293T cells transfected with His-tagged BCR-ABL and GST-tagged RAPSYN. (C) Immunoblot of GST and His following GST pull-down after in vitro incubation of purified His-tagged BCR-ABL and GST or GST-tagged RAPSYN. (D) His-immunoblot of GST immunoprecipitates from HEK293T cells transfected with GST-tagged RAPSYN alone or in combination with His-tagged full-length or truncated BCR-ABL (Δ1: aa 1-927, Δ2: aa 928-2047). (E) Analysis of BCR-ABL neddylation levels in leukemic cells. (F) Analysis of BCR-ABL neddylation levels in leukemic cells treated with MLN4924 or DMSO for 24 h. (G) HA-immunoblot of His-immunoprecipitate from HEK293T cells transfected with His-tagged BCR-ABL and HA-tagged NEDD8 or NEDD8 ΔGG. (H) HA-immunoblot of the His-immunoprecipitate from HEK293T cells transfected with the indicated constructs. (I) Analysis of BCR-ABL neddylation levels in K562 WT, RAPSYN KO and RAPSYN KO with exogenous expression of a RAPSN cDNA cells. (J) HA-immunoblot after immunoprecipitation of His in HEK293T cells transfected with His-tagged BCR-ABL, HA-tagged NEDD8, GFP-tagged WT RAPSYN or C366A RAPSYN. (K) Assessment of BCR-ABL neddylation by RAPSYN in vitro. Purified RAPSYN and BCR-ABL were incubated with APPBP1/UBA3, UBE2M or NEDD8 in in vitro neddylation assay. (L) Analysis of BCR-ABL neddylation levels in excised tumor xenografts from . (M) Verification of BCR-ABL neddylation sites in HEK293T cells transfected with indicated constructs.

    Article Snippet: A 30 μL reaction mixture containing 2 mM ATP-Mg2+ (B-20, R&D), 50 ng E1(APPBP1/UBA3; E-313-25, R&D), 400 ng E2 (UBE2M; E2-656-100, R&D), 0.25 μg NEDD8 (UL-812-500, R&D), 0.35 μg His-BCR-ABL, with or without 4.77 μg RAPSYN was incubated at 37°C for 4 h. Reaction was terminated with SDS-PAGE loading buffer and assayed using immunoblotting.

    Techniques: Immunoprecipitation, Western Blot, Transfection, In Vitro, Incubation, Purification, Construct, Expressing